Emission spectra

From the FRET efficiency data and assuming a Förster radius of 6 nm calculate the donor-acceptor separation for all data points and display this data as a histogram.

1. Please report this exercise as if you were writing up an experimental method. Using the equation below, calculate the emission spectra s for Donor and acceptor molecules with absorption and emission spectra given in the spreadsheet (forster radius data(BB0344).xls). Where Q = quantum yield of D in the absence of A 2 = factor […]

Scroll to top